Vale in the Northern Section - West
First round ties will be played during the week commencing Monday 2 September.
The draw will be split into four sections, as below.
Northern Section - West
Accrington Stanley, Bury, Crewe Alexandra, Fleetwood Town, Oldham Athletic, Port Vale, Shrewsbury Town and Tranmere Rovers.
Northern Section - East
Bradford City, Burton Albion, Hartlepool United, Notts County, Scunthorpe United, Sheffield United, Walsall and Wolverhampton Wanderers.
Southern Section - West
Bristol City, Bristol Rovers, Cheltenham Town, Exeter City, Plymouth Argyle, Portsmouth, Torquay United and Wycombe Wanderers.
Southern Section - East
AFC Wimbledon, Brentford, Colchester United, Dagenham & Redbridge, Gillingham, Leyton Orient, Milton Keynes Dons and Northampton Town.
Meanwhile, the 16 clubs that have been handed a bye for round one have been confirmed as follows.
Northern Section Byes
Carlisle United, Morecambe, Preston North End, Rochdale, Chesterfield, Mansfield Town, Rotherham United and York City.
Southern Section Byes
Crawley Town, Newport County, Oxford United, Swindon Town, Coventry City, Peterborough United, Southend United and Stevenage.